TEST FOR SUGAR

























































  

TEST FOR SUGAR

 

Aim

To detect whether sugar is present in the given sample of urine or not.

Apparatus

Test tube holder, test tube, spirit lamp, pipette, Benedict’s reagent

Procedure

Take 5 ml. of Benedicts reagent in a clean test tube and boil. Add 0.5 ml. (8 drops) of urine in it and mix well. Boil for two minutes again over a spirit lamp. Place the test tube on a stand for 5-10 minutes. If sugar is present, a red or yellow precipitate will appear. A slight green colour or a bluish white precipirate or no change is recorded as -ye. A greenish colour with little yellow deposite is reported as trace (0.5%), green yellow (1%), orange colour (1.5%) and brick red colour (2%).

Note:

The observations may be classified as no change (-ye), greenish colour with little yellow precipitate

- 0.5%), green yellow colour (++ - 1%) and brick red colour (+++ - 2%).

Sample

Observation

Result

I

II

III

Iv

Questions:

1. If blood sugar is normal, but sugar is present in urine, what is your inference?

2. In what all conditions other than Diabetis mellitus is sugar present in urine?

 

 

 

 

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